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Liquid Cultured

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Everything posted by Liquid Cultured

  1. nvm, its not it on my logs, cant remove post so typing this
  2. So cool, thanks for sharing. Just started reading about it and one person uses it to help treat colds. I've been to a few places south and east of Indianapolis and haven't encountered any yet but I don't have that great of a sample size of outings. Did you find it deeper into the woods?
  3. To give a better idea of what I'm talking about with the trash bag technique as I just got around to picking my flush of oysters. The reason you can generally use new egg flats without sterilizing or pasteurization with chemicals because the medium is pretty inert and doesn't aid much in bacterial growth. But since the material is paper pulp and paper is made from trees the mushrooms can create enzymes to break down and use it for nutrients. I also imagine it uses whatever energy was stored in the fruit itself that was used to inoculate these blocks. Had been making dripper bottles of the main chemicals used in testing mushrooms so the label cuttings etc make it look dirty in there. I figured I might as well grab spores while I'm at it since I've gotten a microscope and slides. I like using sterile petri over foil or card stock and once the caps are removed I will cook them or dehydrate. To the reason why I do this is because they will get wrapped with parafilm and put onto a shelf in the hood room. But also, its so when I want to make a spore swab (don't need to fill with water but I suppose you could) or syringes I can fill the petri up with water, swirl it around and then suck a ml or two up in several syringes. I've also found that when you are finished up with doing a bunch of petri work I will empty the agar discs left in the petris, clean them with isopropyl, and then reused for spore prints. Of course you can do this in a ziplock bag, filling with water and all that which is how most store their spore prints but I have an excess of petri. I've also spore printed a few judas ears and will be trying to grow it out on agar this weekend. Anyone can request spores from things I collect and grow for free if we meet or cover shipping. Same goes with the chemical tester solutions.
  4. Created a proton email for anyone who wishes to communicate with me outside the forum without giving out personal numbers etc or those who dont use telegram/messaging apps. LiquidCultured at protonmail dot com. Replace "at" with @ and "dot" with . Helps reduce crawler bots reading the email address and sending me spam.
  5. Supposed to be king oysters but thinking that was not the case, must have mixed up liquid cultures. Had a contamination/moisture issues with the rest of the buckets as I got lazy so only 4 of 10 2gallon buckets fruited. Going to try cloning chanterelles with 12" egg crates as the medium like I'm doing with these oysters. Some of you may know the trash bag method and that's basically what I'm doing. Straw logs work great, straw buckets... not so much. TLDR trash bag tek you'd take either grain spawn or the raw fresh fruit and place in the center of a stack of egg crates or news paper/books after saturating the egg cardboard with RO/distilled sterile water while in the trash bag. Tie off bag, wait 3 or 4 weeks then take the now solid block of mycelium out and put into a fruiting chamber or just open the bag enough for decent air exchange. Done
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  7. These came after the forage but will have them ready to use if my dropper bottles get here before this weekend for the Shade park foray. If anyone wants 5ml droppers of these once I can make up a batch of each let me know and ill bring them free to the next meetup.
  8. All images from my phone. I do have a lot of these on the DSLR in macro in the field but those need processed as I shoot in RAW format, then get the photos from over 30mb each to under 2mb so I can post here. I've also put them up on my telegram chat I link to in another post. When I have them all tidy and clean photos I'll upload to my flickr account so you guys can see full resolution and very close details. I'm dehydrating all the things I forage. Don't plan to eat any until I can get into mycologist led forays from the HMS and know a lot more. Not sure if true chanterelle but not quite smooth and not a jack o lantern. Smell like apricots, well, at least smells like the nasal snuff I have that's apricot. I promise I'm not a hipster haha looked edible, type of button? but haven't started trying to ID. Probably should spend more time reading my books as I feel bad getting home and feeling overwhelmed taking multiple species and not getting to them all or skipping some for whatever reason. Gathered these but from google lens AI guess its not a safe mushroom (Orange mycena or start of jack o lanterns?) I'm not dehydrating This red shelf poly smells amazing, rather soft but firm Found on dead log, no bark Varnish mush? Thought I'd lift the tree stump out of the ground before this came off. This one also smelt good, found near the white polypore 2 picture's up Jelly/wood/judas ear. Found some pristine samples, maybe 10-15 total. Nearly impossible to remove from the downed tree trunk but also smells quite nice
  9. Found a bunch more today at another park. Easily a meal worth. Dehydrating ruins them for cooking later?
  10. Forgot to add the bolete cross section. Instantly blues
  11. I havent eaten anything I've found and probably wont for some time, but these did seem tempting after reading about them. I guess drying them ruins them then, will keep fresh next time. I may try cloning them on agar and see if they are cultivatable.
  12. I haven't tried identifying these yet as I just took them. Well, the bolete I have a clue. I have a nice new macro lens coming this week and its a NIKKOR Z MC 105mm f/2.8 VR S. I have its smaller 50mm brother which is what I used on these images. Camera is a Nikon z7 ii if anyone is interested. Female wolf spider eating her hubby after mating was among the tiny little mushrooms I was trying to get pictures of. Next mushroom up Found this pair of mushrooms one with what appears to be a parasitic fungi on it? Then these pretty guys The boletes
  13. Planning to visit this park august 11th to look for mushrooms as there isn't rain in the forecast for a while after what we just had and i havent been there before. if anyone wanted to tag along or would also be in the area let me know. It will be in the afternoon. Southeastway Park https://maps.app.goo.gl/14xNWcXRyG8AFf4dA
  14. I mentioned green spores as the gills in the photo appeared to have green staining. But that could be from the touch not the spores collecting on the gills. Finding them in multiple ages also helps because some gills might be pink when young then change to black as they mature etc. There are a handful of mushrooms that will fairy ring some in the Amanita genus etc. See my profile for a list of books that come highly recommended in assisting identification and lookalikes. It's best to use multiple book sources to identify. Mushroomexpert.com run by mycologist Michael Kuo has keys for must mushrooms used for identification and is another great source. He is an author of multiple books in my list. The HMS is doing DNA sequencing for mushrooms found during the summer and fall forays. I'm not sure if they offer a paid service for collected elsewhere or otherwise.
  15. Never encountered them in person and was quite the treat to find. Preserved and added to collection.
  16. A spore print can also help; if the spores are green then most likely that but if White then not.
  17. Does this match up to what you overserved? https://plants.ces.ncsu.edu/plants/chlorophyllum-molybdites/
  18. Not sure if this is an important note or not but being a bug guy I've noticed the exact same flat, segmented millipede is at all the sites I find these and the white jellys. They are living under the fungi and nearby bark. If you don't know anything about millipedes and what some do when disturbed you might grab for one of these jelly fungi then bring it up to smell only to be repulsed by the smell of hydrogen cyanide. These flat millipedes and some others produce this chemical as a way of protecting themselves from predators by excreting it from their segments. You will find that mushrooms picked where not many of these insect are actively on that it has a nice faint smell, sometimes buttery. I don't think the concentration of cyanide would harm a human but it's hard to get off your skin smell wise, and I wouldn't imagine it will wash off easily.
  19. I have recently started a telegram group with some family and friends in it where they can post stuff they find etc if anyone is interested. Just let us know this is where you got the link so we know it's not bot spammers. https://t.me/+un8D6QzvqLM0NTcx
  20. Me and the wife are going to try and be at this foray if anyone would like to meet me first in a group setting. I deal with anxiety so if I seem shy that's why. August 13 - Shades State Park Walk - 1pm - 3pm - Led by Stephen Russell
  21. Donated 250 to help with sequencing from forays. I really hope to get to one eventually especially the certification classes but realize im late in season. For those who do go foraging on the day and time of a scheduled foray using inaturalist but couldn't attend the location can we send in samples to have sequenced? Using the printable cards for the event of course.
  22. Thanks found some other interesting mushrooms at the park today, one bright red bean looking cluster with what i think was a tap root. Also ended up ordering some chemicals for testing mushrooms in the future. Ammonia (NH3) Ferrous sulphate 10% Potassium hydroxide 10% Hydrochloric acid 32% Lugol’s Iodine 5%
  23. My name is Kevin. As there are not many forays near me happening and not everyone wants to do those I figured I'd put this line out here. I'm willing to drive within reason to meet up. I'm located in Greenwood, IN. Groups or solo. I'd even be fine just holding the bags, taking macro and profile photos, or even scribbling down your descriptions etc. I know some mushroom foragers can be iffy with new people and their intentions but I'm not interested in this to gain your personal location knowledge or what I can snag for a meal but actually learn how to identify in and out of the field any mushrooms. I've been cultivating indoors off and on for nearly 15 years, I've slowly learned some things over that time but not nearly enough and that's why I'm here. Bad with mycological terminology besides the obvious but I'm learning. I have access to personal home lab stuffs and knowledge including a graduated glassware, lots of mixers, a few autoclaves, pressure cookers, sterile petri packs, a quality 4' long by 2' tall laminar flow hood. I can make my own agars and pour quite well, use a scalpel to do transfers or save a contaminated plate. Perform cold/hot pasteurization techniques. Spore printing, but anyone can do that :). I've gotten single strain isolations from multi-spore plates, only did this a few times mostly for the fun and to see if I could. I really enjoy working with liquid cultures and have made my fair share and lost a fair share too. I don't have any magnification equipment besides my macro lenses for the DSLR but looking to get a microscope once I learn what to get. Current books I've acquired to help with the journey. Illustrated Dictionary of Mycology Mushrooms Demystified by David Arona PB Second Edition(to pair with my pocket version) Mushrooms of the Midwest, Paperback by Kuo, Michael 100 Edible Mushrooms by Kuo, Michael Edible Wild Mushrooms of Illinois & Surrounding States by Gregory M. Mueller and Joe McFarland DK Handbook: Mushrooms The Clearest Recognition Guide by Laessoe, Thomas Simon and Schuster's Guide to Mushrooms Trees of Indiana Field Guide I'm free a lot, work at home, and also a soon to be stay at home dad. I will use this knowledge when the time to homeschool get's here in the coming years. I'm also a homesteader in progress so enjoy organic gardening etc. Canning. Super easy going and laid back when the time is appropriate. Oh, and I don't have access to social "media" so only by phone, text, email, telegram(app).
  24. Found these on a drive around greenwood off in a grassy lot. Didn't smell very inviting and the flakes get everywhere. Had a brown liquid on the caps of the flatter ones that held a lot of the odor. Being new to wild mushroom IDing I can't think what phenolic smells like or other weird smells. Wasn't chemically, but a dirty smell, not mushroomy. I can't recall the name of the bush that smells to me like cat pee, but it had that sort of odor. I didn't notice an egg or cup. Spore print was White, didn't taste it, still way too nervous about doing that even though it's regarded as "safe" so long as you don't swallow the flesh. Narrowed down to either Agaricus sp or Amanta sp using Arona's pocket guide key then moved on from there. Didn't take a cross section but can drag them out of the compost pile but might be gross by now as it's been a few days in the heat. Cap and stalk didn't change color when damaged or rubbed repeatedly.
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