The Flongle is an add-on component to the MinION, which allows for smaller DNA Sequencing runs. This (in theory) means faster and cheaper results. Currently, these units only work with a previous generation of flow cells (R9) rather than the newer (R10.4 - dual readers on channel). We would like to compare both technologies. This will also allow us to be ready to take full advantage of the Flongle promise when R10.4 flow cells are released for the Flongle.
DNA Sequencing with MinION course
Fall 2022 – Likely Saturday, Oct. 22, 2022 (Subject to change)
This course will cover all the basics of DNA sequencing with the MinION. Topics included will be specimen collection and drying, taking tissue, DNA extraction, DNA amplification, DNA sequencing, and data analysis. It will cover all of the benefits, challenges, and limitations of the MinION system with hands-on experience for each participant. This will be a full day course, starting at 9am and continuing into the late evening. The event will be held in the Indianapolis area that has a large conference room or classroom, as well as an abundance of woods nearby. We will do a 2 hour foray in the morning and then spend the rest of the day working the DNA sequencing process. No previous experience is required and every topic will be taught from the beginner perspective.
There will be a tiered admission for registration. Each tier has a limited number of registrants available. The first 5 people to make a donation and comment here: https://m.facebook.com/groups/indianamushrooms/permalink/3135269980082033/ will be able to register at that price tier. That tier is then full. There is a limit of 20 participants across all tiers. For the next 24 hours, registration is limited to the 20 individuals who voted “Yes” in the poll that they would be interested in the course. Registration will then be opened up to others.
Level 1 (Early Bird VIP) - $150 – 5 spots - FULL
Level 2 (Supporter) - $200 – 5 spots - FULL
Level 3 (General Participant) - $250 – 10 spots - FULL
The goals of hosting this course are four-fold. 1.) To test out a workflow that will allow attendees of a large foray to participate in bulk sequencing specimens – to sequence a large number of specimens and to get results in a short timeframe. 2.) To quickly get DNA sequence results from our Summer and Fall 2022 online forays. 3.) To help fund the equipment and supplies that will allow us to perform DNA sequencing locally at a low cost. 4.) Provide registrants with a working-level knowledge of fungal DNA sequencing with MinION.