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Liquid Cultured

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Posts posted by Liquid Cultured

  1. To give a better idea of what I'm talking about with the trash bag technique as I just got around to picking my flush of oysters. The reason you can generally use new egg flats without sterilizing or pasteurization with chemicals because the medium is pretty inert and doesn't aid much in bacterial growth. But since the material is paper pulp and paper is made from trees the mushrooms can create enzymes to break down and use it for nutrients. I also imagine it uses whatever energy was stored in the fruit itself that was used to inoculate these blocks. 

    Had been making dripper bottles of the main chemicals used in testing mushrooms so the label cuttings etc make it look dirty in there. 

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    I figured I might as well grab spores while I'm at it since I've gotten a microscope and slides. I like using sterile petri over foil or card stock and once the caps are removed I will cook them or dehydrate. To the reason why I do this is because they will get wrapped with parafilm and put onto a shelf in the hood room. But also, its so when I want to make a spore swab (don't need to fill with water but I suppose you could) or syringes I can fill the petri up with water, swirl it around and then suck a ml or two up in several syringes. I've also found that when you are finished up with doing a bunch of petri work I will empty the agar discs left in the petris, clean them with isopropyl, and then reused for spore prints. Of course you can do this in a ziplock bag, filling with water and all that which is how most store their spore prints but I have an excess of petri.

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    I've also spore printed a few judas ears and will be trying to grow it out on agar this weekend. Anyone can request spores from things I collect and grow for free if we meet or cover shipping. Same goes with the chemical tester solutions. 

    • Thanks 1
  2. Created a proton email for anyone who wishes to communicate with me outside the forum without giving out personal numbers etc or those who dont use telegram/messaging apps.

    LiquidCultured at protonmail dot com. Replace "at" with @ and "dot" with .

    Helps reduce crawler bots reading the email address and sending me spam.

    • Like 1
  3. Supposed to be king oysters but thinking that was not the case, must have mixed up liquid cultures. 

    Had a contamination/moisture issues with the rest of the buckets as I got lazy so only 4 of 10 2gallon buckets fruited. Going to try cloning chanterelles with 12" egg crates as the medium like I'm doing with these oysters. Some of you may know the trash bag method and that's basically what I'm doing. Straw logs work great, straw buckets... not so much.

    TLDR trash bag tek you'd take either grain spawn or the raw fresh fruit and place in the center of a stack of egg crates or news paper/books after saturating the egg cardboard with RO/distilled sterile water while in the trash bag. Tie off bag, wait 3 or 4 weeks then take the now solid block of mycelium out and put into a fruiting chamber or just open the bag enough for decent air exchange. Done

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    • Like 1
  4. All images from my phone. I do have a lot of these on the DSLR in macro in the field but those need processed as I shoot in RAW format, then get the photos from over 30mb each to under 2mb so I can post here. I've also put them up on my telegram chat I link to in another post. When I have them all tidy and clean photos I'll upload to my flickr account so you guys can see full resolution and very close details. I'm dehydrating all the things I forage. Don't plan to eat any until I can get into mycologist led forays from the HMS and know a lot more.

    Not sure if true chanterelle but not quite smooth and not a jack o lantern. Smell like apricots, well, at least smells like the nasal snuff I have that's apricot. I promise I'm not a hipster haha 

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    looked edible, type of button? but haven't started trying to ID. Probably should spend more time reading my books as I feel bad getting home and feeling overwhelmed taking multiple species and not getting to them all or skipping some for whatever reason. 

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    Gathered these but from google lens AI guess its not a safe mushroom (Orange mycena or start of jack o lanterns?) I'm not dehydrating

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    This red shelf poly smells amazing, rather soft but firm

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    Found on dead log, no bark

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    Varnish mush? Thought I'd lift the tree stump out of the ground before this came off.

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    This one also smelt good, found near the white polypore 2 picture's up

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    Jelly/wood/judas ear. Found some pristine samples, maybe 10-15 total.

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    Nearly impossible to remove from the downed tree trunk but also smells quite nice

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  5. I haven't tried identifying these yet as I just took them. Well, the bolete I have a clue. I have a nice new macro lens coming this week and its a NIKKOR Z MC 105mm f/2.8 VR S. I have its smaller 50mm brother which is what I used on these images. Camera is a Nikon z7 ii if anyone is interested.

    Female wolf spider eating her hubby after mating was among the tiny little mushrooms I was trying to get pictures of.

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    Next mushroom up

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    Found this pair of mushrooms one with what appears to be a parasitic fungi on it?

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    Then these pretty guys

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    The boletes

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    • Thanks 1
  6. I mentioned green spores as the gills in the photo appeared to have green staining. But that could be from the touch not the spores collecting on the gills. Finding them in multiple ages also helps because some gills might be pink when young then change to black as they mature etc. There are a handful of mushrooms that will fairy ring some in the Amanita genus etc. See my profile for a list of books that come highly recommended in assisting identification and lookalikes. It's best to use multiple book sources to identify. Mushroomexpert.com run by mycologist Michael Kuo has keys for must mushrooms used for identification and is another great source. He is an author of multiple books in my list.

    The HMS is doing DNA sequencing for mushrooms found during the summer and fall forays. I'm not sure if they offer a paid service for collected elsewhere or otherwise.

  7. Not sure if this is an important note or not but being a bug guy I've noticed the exact same flat, segmented millipede is at all the sites I find these and the white jellys. They are living under the fungi and nearby bark. If you don't know anything about millipedes and what some do when disturbed you might grab for one of these jelly fungi then bring it up to smell only to be repulsed by the smell of hydrogen cyanide. These flat millipedes and some others produce this chemical as a way of protecting themselves from predators by excreting it from their segments. You will find that mushrooms picked where not many of these insect are actively on that it has a nice faint smell, sometimes buttery. I don't think the concentration of cyanide would harm a human but it's hard to get off your skin smell wise, and I wouldn't imagine it will wash off easily.

  8. Donated 250 to help with sequencing from forays. I really hope to get to one eventually especially the certification classes but realize im late in season. For those who do go foraging on the day and time of a scheduled foray using inaturalist but couldn't attend the location can we send in samples to have sequenced? Using the printable cards for the event of course. 

  9. 15 hours ago, Bruce said:

    That's what they look like to me.

    Bruce

    Thanks :) found some other interesting mushrooms at the park today, one bright red bean looking cluster with what i think was a tap root. Also ended up ordering some chemicals for testing mushrooms in the future.

    • Ammonia (NH3)
    • Ferrous sulphate 10%
    • Potassium hydroxide 10%
    • Hydrochloric acid 32%
    • Lugol’s Iodine 5%
  10. My name is Kevin. As there are not many forays near me happening and not everyone wants to do those I figured I'd put this line out here. I'm willing to drive within reason to meet up. I'm located in Greenwood, IN. Groups or solo. I'd even be fine just holding the bags, taking macro and profile photos, or even scribbling down your descriptions etc. I know some mushroom foragers can be iffy with new people and their intentions but I'm not interested in this to gain your personal location knowledge or what I can snag for a meal but actually learn how to identify in and out of the field any mushrooms. I've been cultivating indoors off and on for nearly 15 years, I've slowly learned some things over that time but not nearly enough and that's why I'm here. Bad with mycological terminology besides the obvious but I'm learning.

    I have access to personal home lab stuffs and knowledge including a graduated glassware, lots of mixers, a few autoclaves, pressure cookers, sterile petri packs, a quality 4' long by 2' tall laminar flow hood. I can make my own agars and pour quite well, use a scalpel to do transfers or save a contaminated plate. Perform cold/hot pasteurization techniques. Spore printing, but anyone can do that :). I've gotten single strain isolations from multi-spore plates, only did this a few times mostly for the fun and to see if I could. I really enjoy working with liquid cultures and have made my fair share and lost a fair share too. I don't have any magnification equipment besides my macro lenses for the DSLR but looking to get a microscope once I learn what to get. 

    Current books I've acquired to help with the journey.

    • Illustrated Dictionary of Mycology
    • Mushrooms Demystified by David Arona PB Second Edition(to pair with my pocket version)
    • Mushrooms of the Midwest, Paperback by Kuo, Michael
    • 100 Edible Mushrooms by Kuo, Michael
    • Edible Wild Mushrooms of Illinois & Surrounding States by Gregory M. Mueller and Joe McFarland
    • DK Handbook: Mushrooms The Clearest Recognition Guide by Laessoe, Thomas
    • Simon and Schuster's Guide to Mushrooms
    • Trees of Indiana Field Guide

    I'm free a lot, work at home, and also a soon to be stay at home dad. I will use this knowledge when the time to homeschool get's here in the coming years. I'm also a homesteader in progress so enjoy organic gardening etc. Canning. Super easy going and laid back when the time is appropriate. 

    Oh, and I don't have access to social "media" so only by phone, text, email, telegram(app).

     

  11. Found these on a drive around greenwood off in a grassy lot. Didn't smell very inviting and the flakes get everywhere. Had a brown liquid on the caps of the flatter ones that held a lot of the odor. Being new to wild mushroom IDing I can't think what phenolic smells like or other weird smells. Wasn't chemically, but a dirty smell, not mushroomy. I can't recall the name of the bush that smells to me like cat pee, but it had that sort of odor. I didn't notice an egg or cup. Spore print was White, didn't taste it, still way too nervous about doing that even though it's regarded as "safe" so long as you don't swallow the flesh. Narrowed down to either Agaricus sp or Amanta sp using Arona's pocket guide key then moved on from there. Didn't take a cross section but can drag them out of the compost pile but might be gross by now as it's been a few days in the heat. Cap and stalk didn't change color when damaged or rubbed repeatedly.

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  12. On 1/22/2022 at 4:08 PM, digiren said:

    I know this is super late but I think it also could have been amber jelly / Excidia Recisa I have one of these growing on a willow in my yard. They look fairly similar and are also edible. Anyone have tips on telling the two apart? I’m just getting into mycology so sorry if this is off topic or I’m reviving a thread that’s way too old haha

    Also super late as I just foraged my first Judas ears today, well, yesterday evening. The one sure way to pick the correct judas/jelly/wood ear is if its on Elder Trees, or debris of elder trees. From reading they may also grow on other trees but elder is the fungi's favorite and no other lookalikes grow on elder trees.

    As for cooking, it's widely used by east asians to thicken soups or rehydrating them in something flavorful as they take on external flavor really well and get plump. You could try going to an asian grocery and checking them out and compare.

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